How can the Multipette/Repeater M4 be calibrated?

To calibrate Multipettes/Repeaters in accordance with EN ISO 8655, three different volumes with ten measured values each have to be checked. These three volumes are the nominal volume (= the maximum volume to be dispensed), 50% of the nominal volume and 10% of the nominal volume of the Combitips advanced used. The checking procedure, the test conditions, and the limits in accordance with EN ISO 8655 are described in our "Eppendorf SOP for checking pipettes". The SOP is available on our web page.

Can the M4 Multipette/Repeater M4 be autoclaved?

No, the Multipette/Repeater M4 cannot be autoclaved. We recommend wiping the device with a cloth that has been moistened with a disinfectant (e.g. 60% isopropanol, 12% bleach). Please ensure that no moisture can get into the device during cleaning.

Can Combitips advanced advanced be partially filled as well?

Yes, with the Multipette/Repeater M4, Combitips advanced can be partially filled as well. To ensure accurate dosing, it is mandatory that the pistons of the Combitips advanced are moved to a defined position after aspiration. Discard the steps by pressing the operating lever until the display stops flashing and “steps 0” is displayed. If the selection dial position is below 4, several steps must be discarded.

Why can the Multipette/Repeater M4 only be operated with Combitips advanced?

When using a positive displacement system, the dispenser and the dispenser tip must be perfectly matched. This will ensure that the high demands with regard to precision and accuracy are reliably met. Combitips advanced are perfectly matched to Eppendorf Multipettes and, therefore, to the Multipette/Repeater M4. In combination with the center sleeve of the Multipette M4, accurate dosing for wall dispensing is supported in particular.

What is the maximum number of dispensing steps that can be carried out with the Multipette/Repeater M4?

In position 0.5, 100 dispensing steps can be performed with the Multipette/repeater M4.

What is the volume range covered by the Multipette/Repeater M4 when Combitips advanced are used?

Eppendorf offers 9 different sizes of Combitips advanced. This enables the Multipette/Repeater M4 to cover a dispensing volume of 1 µL - 10 mL.

Which chemicals can be dispensed with the Multipette/Repeater M4?

During the development of the Multipette/Repeater M4, the use of materials with a high chemical resistance was a priority. Of course, the chemical resistance of the Combitips advanced is also relevant here. You can find a detailed list of the chemical resistance for both products in the corresponding operating manual or in the instructions for use.

Can you dispense 2 µL in a free jet?

With the Combitip advanced 0.1 mL in selector dial position 1 and the Combitip advanced 0.2 mL in selector dial position 0.5, a volume of 2 µL can be dispensed in a free jet. All volumes can be dispensed in a free jet, if handled correctly. If you want to carry out all dispensing steps as a free jet, the reverse stroke should be dispensed in a free jet as well. Please note that the technical data document recommends wall dispensing.

How can I extend the life of the battery as much as possible?

The life of the battery very much depends on the type and frequency of use. To extend the life of the battery as much as possible, the Multipette M4 goes into sleep mode when it is not in use. You should also remove the Combitips advanced immediately after use and not store the Multipette M4 with the Combitip advanced inserted to extend the life of the battery.

How long will the battery last (for the use of the display) after the battery symbol appears?

If the battery symbol only shows with one segment, the battery capacity is very low. A few more dosing series can be carried out. Have a replacement battery to hand. As soon as the empty battery symbol appears, the battery should be replaced.

Does the Multipette/Repeater M4 also work without a battery?

Yes. The Multipette/Repeater M4 also works without a battery. The precision of the dosing is due to the reliable mechanics of the Multipette/Repeater M4. It will not be affected if the battery fails. To help with the selection of the correct dispensing volume, a volume table is included in the short instructions and in the operating manual. In addition, the Combitips advanced indicate the dispensing volume in selector dial position 1. This volume can be multiplied with the selector dial position to calculate the set dispensing volume.

How much does the Multipette/Repeater M4 weigh?

The Multipette/Repeater M4 only weighs 105 g.

What does the “PhysioCare concept” symbol on the Multipette/Repeater M4 mean?

The symbol means that the Multipette/Repeater M4 has been developed in accordance with the considerations of the Eppendorf PhysioCare concept. With its three areas, the concept includes the whole range of ergonomics, not just a single element. Instead of focusing on a particular product feature, the products with the PhysioCare symbol provide a holistic solution. Further details on the PhysioCare Concept ® are available at http://physiocare.eppendorf.com.

Does a specific dispensing angle need to be observed during dispensing?

Yes. If the Multipette/Repeater M4 is held at an angle that is too flat (< 45°C), dosing accuracy is affected. Hold the device as upright as possible, but in a position that is comfortable for you. Even when dispensing the last step, the Multipette/Repeater M4 should be held as straight as possible.

What does “steps” on the display mean?

The Multipette/Repeater M4 has a step counter. If a Combitip advanced is used alongside “steps” (here: dispensing steps), the number of dispensing steps that can be carried out with a full Combitip advanced is displayed. During dispensing, the number of dispensing steps that have already been carried out are displayed. When the selector dial position is adjusted, the number of dispensing steps that can still be carried out is displayed.

Can the step counter also count the dispensing steps if the Combitips advanced are only partially filled?

Yes, the step counter also counts the dispensing steps that were carried out when the Combitip advanced is only partially filled once. As with a full Combitip, the reverse stroke must be carried out first (display flashes). Please note with this type of filling that the step counter continues to count beyond the residual stroke lock.

Can the step counter also count the dispensing steps if the Combitip advanced is filled several times?

Yes, the step counter also counts the dispensing steps when the Combitip advanced is filled to capacity several times.

How are the Combitips advanced ejected?

To eject the Combitips advanced, push the lower filling lever down as far as it will go (dispensing the remaining volume). Then the Combitip advanced can be ejected without touching it, by pressing the upper operating lever.

Is it possible to attach the Multipette/Repeater M4 to a wall?

Yes. The Multipette/Repeater M4 comes with a holder which can be inserted in a pipette carousel or it can be fixed to a clean glass or ceramic surface using the enclosed adhesive strip.

How do I determine the volume values when calibrating gravimetrically?

The measuring values of the analysis scales are multiplied by the correction factor Z (see SOP pipettes). This takes into account the density of the water at the relevant temperature and the relevant air.

What do the terms random error and systematic error mean in a pipette calibration?

The systematic error describes the deviation of the dispensed volume from the nominal volume or from the selected volume of the piston stroke device, and is determined by establishing the mean value of (usually) 10 measurements. The random error describes the mean variation of the dispensed volumes from the mean value of the dispensed volumes, and is determined by calculating the repeat standard deviation of (usually) 10 measurements.

Why are the Z factors listed in our SOP different from factors which can be derived from density reference values (of water)?

The Z factor is included in EN ISO 8655-6 and was incorporated into our SOP. As we understand it, it was incorporated in the standard by the Physikalisch Technische Bundesanstalt in Braunschweig. The Z factor describes the density of water at a particular temperature using a 5th degree polynomial. Furthermore, the density of water is corrected by the density of air (derived from air pressure) and a fixed correction value for balances. These corrections are the key reasons for the deviation from density values in various books of tables.

What does EN ISO 8655 cover?

ISO 8655 addresses the needs of suppliers, test houses, and users and specifies the general requirements for piston-operated liquid handling devices. It is structured in seven chapters: ISO 8655-1: Terminology, general requirements, and user recommendations ISO 8655-2: Piston pipettes ISO 8655-3: Piston burettes ISO 8655-4: Dilutors ISO 8655-5: Dispensers ISO 8655-6: Gravimetric method for the determination of measurement error ISO 8655-7: Non-gravimetric methods for assessment of equipment performance (titration, photometry)

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Multipette^® M4 - Multi-Dispenser Pipette

The Multipette® M4 multi-dispenser (repeater pipette) helps you perform long, repetitive pipetting tasks with ease

The Multipette M4® multi-dispenser with advanced interface and sensor

Side view of the Multipette® M4 multi-dispenser pipette from Eppendorf

The range of Combitips® advanced dispenser tips offer a wide spectrum of possibilities

Multipette® M4 multi-dispenser with an assortment of fitting Combitips® advanced positive displacement pipette tips

Store your Multipette® M4 multi-dispenser securely on a Pipette Carousel 2 or Charger Carousel 2 by Eppendorf

Pipette Holder 2 for Multipette® M4 multi-dispenser pipette from Eppendorf

Multipette® M4 Multi-Dispenser Held By Scientist (Product Close-Up)

Female and Male Scientists Working with Multipette® M4 and Eppendorf Conical Tubes

ACT label of sustainability certification for Multipette® M4 starter kit

Product Information

Say goodbye to tedious, time-consuming serial pipetting! The Multipette M4 pipette from Eppendorf is ideal for repetitive dispensing tasks in long series, eliminating the need for repeated liquid aspiration. Paired with Combitips^® advanced dispenser tips, the Multipette effortlessly handles viscous, volatile or foaming liquids that pose challenges to traditional pipettes. The hermetically sealed tips in the Multipette/Combitips positive displacement pipetting system ensure a high level of safety when working with infectious, toxic, or radioactive liquids.

Show more Product Information

You will find additional download material at the bottom of this page

Product Information

Technical Data

Applications

Features

Aspirate liquid once and dispense up to 100 times without tip refill

The Multipette M4 mechanical multi-dispenser pipette allows you to aspirate liquid once and dispense up to 100 times, making it the ideal precision instrument for long, repetitive pipetting tasks, such as tube or plate filling, and aliquoting reagents. Liquid is aspirated and dispensed by positive displacement, which means there is no air cushion that may be affected by liquid sample properties. This ensures that the correct volume is always dispensed - regardless of the density, viscosity and volatility of the liquid.
The matching Combitips^® advanced and ViscoTip^® positive displacement dispenser tips are automatically recognized - eliminating time consuming volume calculations - and are hermetically sealed to provide a high level of user, instrument and sample safety. Ideal for handling infectious, toxic or radioactive liquids.

Eppendorf pioneered the Multipette/Combitips multi-dispenser system in 1978 and improved it several times since. It was the very first pipetting system allowing users to fill the tip once and dispense multiple times. The concept remains a driver for efficient liquid handling and a staple in many labs working with plates or series of tubes. In 2024, our Multipette M4 multi-dispenser was awarded the ACT^® Environmental Impact Factor Label along with Combitips advanced dispenser tips, showcasing our dedication to eco-conscious manufacturing and assisting customers in taking environmentally informed purchase decisions.

A repetitive pipette with advanced interface and sensor control for easy operation

Step counter
• Displays the number of executed dispensing steps

Volume selection dial
• 20 different volume settings for every size of Combitips advanced

Motion sensor
Even if a Combitips advanced dispenser tip is left attached, the Multipette M4 switches off when not in use to save power. When moved again, it switches back on automatically, while still “remembering” the number of executed dispensing steps for increased efficiency.

Combitips advanced attachment sensor
• Automatic Combitips advanced tip recognition and volume display
• Ensures a secure connection between Combitips advanced and Multipette M4

Make laborious, time-consuming serial pipetting tasks more comfortable

With its easy-to-reach and easily adjustable volume selection dial, the Multipette M4 makes repetitive pipetting tasks manageable. A hand rest supports a relaxed, ergonomic hand position and ease-of-use, ensuring the Multipette M4 provides a greater level of comfort over the day.

The ergonomically shaped operating lever both fills and dispenses the liquid, and also ejects theCombitips advanced dispenser tips, with one-handed operation.

How to use a Multipette M4 repetitive pipette?

The Multipette M4 is extremely easy to use: Just insert a compatible dispenser tip (Combitips advanced or ViscoTip) into the M4 while pushing the operating lever. The pipette automatically recognizes the tip size, and you can directly select the volume and steps needed for your application. The volume can even be changed between dispensing steps. Place the tip into the liquid sample and gently push the operating lever to aspirate the total volume to be dispensed. Push the operating lever once to trigger a reverse stroke needed to maximize accuracy. Afterwards, you can start dispensing into your target vessel(s) by pushing the operating lever slowly as far as it will go. Learn more in our Quick Start Guide.

Convenient storage options for your multi-dispenser & pipettes

Our Multipette M4 pipette holders for wall mounting or the Eppendorf Pipette Holder System can be affixed to virtually any surface or integrated with either our charging or non-charging manual pipette carousel stands. Alternatively, one Multipette M4 multi-dispenser can be stored in the Pipette Stand 2 by Eppendorf.

Disposal of packaging and the dispenser at the end of its lifecycle

The packaging of Multipette M4 dispensers contains different materials, including cardboard and plastic foil. Our Multipette packaging cardboard material consists of approx. 92 % recycling material. Please support the global sustainability initiative of recycling valuable raw material by collecting the cardboard and disposing of it in the appropriate collection container at your organization. In respect to the wrapping foils made of LD-PE, we recommend to select a dedicated recycling partner where PE material can be recycled.

Eppendorf liquid handling instruments are designed for many years of use – should the need arise to dispose of your Multipette however, please note that it may potentially be contaminated and includes electronic components and a battery. Please contact your local biosafety officer and/or your local waste management for requirements and best practices concerning electronic and lab waste. You may use the Eppendorf Decontamination Certificate as guidance regarding possible documentation requirements.

Read Less

Specifications	Multipette^® M4 Starter Kit - 4982000314	Multipette^® M4 - 4982000012

ACT label	yes	yes
Product type	Dispensers	Dispensers
Pipetting type	Positive displacement system	Positive displacement system
Number of channels	1-channel	1-channel
Volume range	1 µL – 10 mL	1 µL – 10 mL
Operating mode	mechanical	mechanical
Random measurement error	see Combitips advanced	see Combitips advanced
Systematic measurement error	see Combitips advanced	see Combitips advanced
Autoclavable	no	no
Battery type	Button cell	Button cell
Consumables	Combitips^® advanced, ViscoTip^®	Combitips^® advanced, ViscoTip^®
Open system (third party consumables)	no	no
Delivery content	Multi-Dispenser, Battery (inserted), Operating manual, Certificate, Combitips^® advanced Box 2.0, Combitips^® advanced assortment pack (1 tip of each size), Pipette Holder 2	Multi-Dispenser, Battery (inserted), Operating manual, Certificate, Combitips advanced tip 2.5 mL, Pipette Holder 2

Downloads: Multipette^® M4

Brochure

Count on it!

The Multipette^® M4 and Combitips^® advanced pipetting system: simple, stress-free and ergonomic

PDF 2.3MB

English

Selection Guide

Get It Right! Selection Chart for Liquid Handling Instruments & Tips

Find the most suitable pipetting system

PDF 1.1MB

English

Brochure

Pipette Service Done Right

Eppendorf Pipette Calibration

Combitips^® advanced dispenser tips from Eppendorf

PDF 2.5MB

English

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Multipette^® M4 - Multi-Dispenser Pipette

Product Information

Product Information

Technical Data

Applications

Features

Aspirate liquid once and dispense up to 100 times without tip refill

A repetitive pipette with advanced interface and sensor control for easy operation

Make laborious, time-consuming serial pipetting tasks more comfortable

How to use a Multipette M4 repetitive pipette?

Convenient storage options for your multi-dispenser & pipettes

Disposal of packaging and the dispenser at the end of its lifecycle

Downloads: Multipette^® M4